Arabidopsis GT34 family contains five xyloglucan α-1,6-xylosyltransferases
Article first published online: 6 JUN 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 195, Issue 3, pages 585–595, August 2012
How to Cite
Vuttipongchaikij, S., Brocklehurst, D., Steele-King, C., Ashford, D. A., Gomez, L. D. and McQueen-Mason, S. J. (2012), Arabidopsis GT34 family contains five xyloglucan α-1,6-xylosyltransferases. New Phytologist, 195: 585–595. doi: 10.1111/j.1469-8137.2012.04196.x
- Issue published online: 9 JUL 2012
- Article first published online: 6 JUN 2012
- Received: 22 April 2012, Accepted: 23 April 2012
Fig. S1 Mass spectra analysis of XXT4 reaction products demonstrating xylosyltransferase activity towards cellohexaose substrate.
Fig. S2 Illustration of T-DNA insertion positions in the xxt mutants used in this study.
Fig. S3 Identification of homozygous T-DNA insertion lines of the Arabidopsis GT34s by PCR amplification.
Fig. S4 Identification of double knock-out lines.
Table S1 Oligonucleotide primers for PCR amplification of nontransmembrane region coding sequences of GT34s for GST-GT34 constructs
Table S2 Oligonucleotide primers for PCR amplification of the five XXT 5’ upstream regions
Table S3 Oligonucleotide primers for RT-PCR amplification of XXT genes
Table S4 Forward and reverse PCR primers for T-DNA insertion homozygous line screening
Table S5 Oligonucleotide primers for RT-PCR amplification of T-DNA insertions disrupting Arabidopsis XXT genes
Table S6 Combinations of UDP-[14C]sugar substrates and UDP-sugar/mono/oligo/polysaccharide acceptors used in assays for screening of Arabidopsis GT34 enzyme activity
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