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Fig. S1 Mass spectra analysis of XXT4 reaction products demonstrating xylosyltransferase activity towards cellohexaose substrate.

Fig. S2 Illustration of T-DNA insertion positions in the xxt mutants used in this study.

Fig. S3 Identification of homozygous T-DNA insertion lines of the Arabidopsis GT34s by PCR amplification.

Fig. S4 Identification of double knock-out lines.

Table S1 Oligonucleotide primers for PCR amplification of nontransmembrane region coding sequences of GT34s for GST-GT34 constructs

Table S2 Oligonucleotide primers for PCR amplification of the five XXT 5’ upstream regions

Table S3 Oligonucleotide primers for RT-PCR amplification of XXT genes

Table S4 Forward and reverse PCR primers for T-DNA insertion homozygous line screening

Table S5 Oligonucleotide primers for RT-PCR amplification of T-DNA insertions disrupting Arabidopsis XXT genes

Table S6 Combinations of UDP-[14C]sugar substrates and UDP-sugar/mono/oligo/polysaccharide acceptors used in assays for screening of Arabidopsis GT34 enzyme activity

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