A sensitive LC-ESI-Q-TOF-MS method reveals novel phytosiderophores and phytosiderophore–iron complexes in barley
Article first published online: 18 JUN 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 195, Issue 4, pages 951–961, September 2012
How to Cite
Tsednee, M., Mak, Y.-W., Chen, Y.-R. and Yeh, K.-C. (2012), A sensitive LC-ESI-Q-TOF-MS method reveals novel phytosiderophores and phytosiderophore–iron complexes in barley. New Phytologist, 195: 951–961. doi: 10.1111/j.1469-8137.2012.04206.x
- Issue published online: 2 AUG 2012
- Article first published online: 18 JUN 2012
- Received: 5 March 2012, Accepted: 8 May 2012
- barley (Hordeum vulgare);
- iron deficiency;
- phytosiderophore (PS);
- root exudates
- •The direct analysis of phytosiderophores (PSs) and their metal complexes in plants is critical to understanding the biological functions of different PSs. Here we report on a rapid and highly sensitive liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (LC-ESI-Q-TOF-MS) method for the direct and simultaneous determination of free PSs and their ferric complexes in plants.
- •In addition to previously reported PSs – deoxymugineic acid (DMA), mugineic acid (MA) and epihydroxymugineic acid (epi-HMA) – two more PSs, avenic acid (AVA) and hydroxyavenic acid (HAVA), were identified by this method in roots of Hordeum vulgare cv Himalaya and in root exudates under iron (Fe) deficiency.
- •The two identified PSs could be responsible for Fe acquisition under Fe deficiency because of their relative abundance and ability to form ferric complexes in secreted root exudates.
- •This LC-ESI-Q-TOF-MS method greatly facilitates the identification of free PSs and PS–Fe complexes in one plant sample.