A sensitive LC-ESI-Q-TOF-MS method reveals novel phytosiderophores and phytosiderophore–iron complexes in barley

Authors

  • Munkhtsetseg Tsednee,

    1. Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
    2. Molecular and Biological Agricultural Sciences, Taiwan International Graduate Program, National Chung-Hsing University and Academia Sinica, Taipei, Taiwan
    3. Graduate Institute of Biotechnology and Department of Life Sciences, National Chung-Hsing University, Taichung, Taiwan
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  • Yit-Wai Mak,

    1. Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
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  • Yet-Ran Chen,

    1. Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
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  • Kuo-Chen Yeh

    1. Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
    2. Molecular and Biological Agricultural Sciences, Taiwan International Graduate Program, National Chung-Hsing University and Academia Sinica, Taipei, Taiwan
    3. Graduate Institute of Biotechnology and Department of Life Sciences, National Chung-Hsing University, Taichung, Taiwan
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Author for correspondence:
Kuo-Chen Yeh
Tel: +886 2 2787 2056
Email: kcyeh@gate.sinica.edu.tw

Summary

  • The direct analysis of phytosiderophores (PSs) and their metal complexes in plants is critical to understanding the biological functions of different PSs. Here we report on a rapid and highly sensitive liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (LC-ESI-Q-TOF-MS) method for the direct and simultaneous determination of free PSs and their ferric complexes in plants.
  • In addition to previously reported PSs – deoxymugineic acid (DMA), mugineic acid (MA) and epihydroxymugineic acid (epi-HMA) – two more PSs, avenic acid (AVA) and hydroxyavenic acid (HAVA), were identified by this method in roots of Hordeum vulgare cv Himalaya and in root exudates under iron (Fe) deficiency.
  • The two identified PSs could be responsible for Fe acquisition under Fe deficiency because of their relative abundance and ability to form ferric complexes in secreted root exudates.
  • This LC-ESI-Q-TOF-MS method greatly facilitates the identification of free PSs and PS–Fe complexes in one plant sample.

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