Initial characterization of shade avoidance response suggests functional diversity between Populus phytochrome B genes
Article first published online: 3 SEP 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 196, Issue 3, pages 726–737, November 2012
How to Cite
Karve, A. A., Jawdy, S. S., Gunter, L. E., Allen, S. M., Yang, X., Tuskan, G. A., Wullschleger, S. D. and Weston, D. J. (2012), Initial characterization of shade avoidance response suggests functional diversity between Populus phytochrome B genes. New Phytologist, 196: 726–737. doi: 10.1111/j.1469-8137.2012.04288.x
- Issue published online: 9 OCT 2012
- Article first published online: 3 SEP 2012
- Manuscript Accepted: 20 JUL 2012
- Manuscript Received: 31 MAY 2012
- Genomic Science Program
- US Department of Energy
- Office of Science
- Biological and Environmental Research
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Fig. S1 Map of binary vector pAGW560 used for transformation of Arabidopsis and Populus.
Fig. S2 Effect of white light (WL) treatment on the expression of candidate Populus shade response genes.
Fig. S3 qPCR analysis of the candidate shade response genes.
Fig. S4 Number of genes identified as being significantly up-regulated (> 3 loge2-fold) or down-regulated (< −3 loge2-fold) after 1 and/or 4 h of low R/FR light treatment.
Fig. S5 qPCR validation of transgenic Populus deltoides lines overexpressing PdPHYB1 (PHYB1OE) or PdPHYB2 (PHYB2OE), or both (PHYB1B2OE).
Fig. S6 Amino acid sequence alignment for predicted Populus trichocarpa PHYB1 and PHYB2 with Arabidopsis thaliana PHYB protein.
Table S1 List of primers used to clone P. trichocarpa (Pt) and P. deltoides (Pd) phytochromes
Table S2 Details of genes used in qPCR and RT-PCR analysis
Table S3 List of the transcripts identified by 454-analysis Populus trichocarpa leaf cDNA after no (control) or 1 h (PtPhy1) or 4 h (PtPhy4) of WL + FR light treatment
Table S4 qPCR validation of 454-analysis
Table S5 List of genes identified by 454-analysis as being up-regulated > 3 loge2-fold after exposure to WL + FR light
Table S6 List of genes identified by 454-analysis as being down-regulated < −3 loge2-fold after exposure to WL + FR light