These authors contributed equally to this work.
An imaging method for oxygen distribution, respiration and photosynthesis at a microscopic level of resolution
Article first published online: 17 SEP 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 196, Issue 3, pages 926–936, November 2012
How to Cite
Tschiersch, H., Liebsch, G., Borisjuk, L., Stangelmayer, A. and Rolletschek, H. (2012), An imaging method for oxygen distribution, respiration and photosynthesis at a microscopic level of resolution. New Phytologist, 196: 926–936. doi: 10.1111/j.1469-8137.2012.04295.x
- Issue published online: 9 OCT 2012
- Article first published online: 17 SEP 2012
- Manuscript Accepted: 25 JUL 2012
- Manuscript Received: 22 JUN 2012
- Bundesministerium für Wirtschaft und Technologie
- fluorescent optical sensors;
- oxygen imaging;
- planar sensor;
- Biological samples are far from homogeneous, with complex compartmentation being the norm. Major physiological processes such as respiration do not therefore occur in a uniform manner within most tissues, and it is currently not possible to image its gradients in living plant tissues.
- A compact fluorescence ratiometric-based device is presented here, consisting of an oxygen-sensitive foil and a USB (universal serial bus) microscope. The sensor foil is placed on the sample surface and, based on the localized change in fluorescence signal over time, information about the oxygen consumption (respiration) or evolution (photosynthesis) can be obtained.
- Using this imaging technique, it was possible to demonstrate the spatial pattern of oxygen production and consumption at a c. 20-μm level of resolution, and their visualization in the rhizosphere, stem and leaf, and within the developing seed. The oxygen mapping highlighted the vascular tissues as the major stem sink for oxygen. In the leaf, the level of spatial resolution was sufficient to visualize the gas exchange in individual stomata.
- We conclude that the novel sensor set-up can visualize gradients in oxygen-consuming and producing processes, thereby facilitating the study of the spatial dynamics of respiration and photosynthesis in heterogeneous plant tissues.