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Fig. S1 Composition of mature seeds from the MtP330S and wild-type genotypes.

Fig. S2 Protease activity tests for the wild-type and MtP330S alleles.

Fig. S3 Expression profiles across plant tissues of the Medicago truncatula and Arabidopsis subtilase genes from the SBT1 subgroup.

Fig. S4 Expression data across seed tissues for the eight Arabidopsis subtilases from the SBT1 subgroup.

Fig. S5 Seed weight variations between wild-type and the heterozygous (Het) and homozygous (HO) mutant plants.


Table S1 RNA-Seq data for the PsSBT1.1 gene in 12 organs at five developmental stages and under two nutrition conditions

Table S2 List of dCaps primers used for genotyping the pea and Medicago truncatula mutants

Table S3 In silico mapping of genes between the genetic markers MTE30 and MTE32 which are anchored to the physical map of Medicago truncatula

Table S4 SIFT (Sorting Intolerant From Tolerant) predictions for the amino acid changes identified by TILLING (Targeting Induced Local Lesions in Genomes)

Table S5 Microscopic observations of mature seeds from the MtP330S TILLING (Targeting Induced Local Lesions in Genomes) mutant and the wild-type form of MtSBT1.1