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Fig. S1 The effects of silicon (Si) supply and grain load on the response of the net CO2 assimilation rate (A) to chloroplast CO2 concentration (Cc) of two rice genotypes (cv ‘Oochikara’ (WT) and the lsi1 mutant defective for Si uptake) grown in nutrient solutions.

Fig. S2 The effects of silicon (Si) supply and grain load on the mesophyll conductance (gm) estimated using the curve-fitting approach (Ethier method) of two rice genotypes (cv ‘Oochikara’ (WT) and the lsi1 mutant defective for Si uptake) grown in nutrient solutions, and the relationship between estimates of gm (Harley and Ethier approaches).

Table S1 Relative metabolite contents in flag leaves of two rice genotypes

Table S2 Over-representation analysis of the canonical variable loadings of metabolites with a main impact on the variance of the dataset

Table S3. Pairwise correlation coefficients, with their corresponding P values, calculated between the contents of Si and all other metabolites