The D3 F-box protein is a key component in host strigolactone responses essential for arbuscular mycorrhizal symbiosis
Article first published online: 1 OCT 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 196, Issue 4, pages 1208–1216, December 2012
How to Cite
Yoshida, S., Kameoka, H., Tempo, M., Akiyama, K., Umehara, M., Yamaguchi, S., Hayashi, H., Kyozuka, J. and Shirasu, K. (2012), The D3 F-box protein is a key component in host strigolactone responses essential for arbuscular mycorrhizal symbiosis. New Phytologist, 196: 1208–1216. doi: 10.1111/j.1469-8137.2012.04339.x
- Issue published online: 5 NOV 2012
- Article first published online: 1 OCT 2012
- Manuscript Accepted: 10 AUG 2012
- Manuscript Received: 25 JUN 2012
- KAKENHI. Grant Numbers: 23128513, 23657044, 24228008, 23370025, 24114010
- arbuscular mycorrhiza;
- F-box protein;
- tiller outgrowth
- Arbuscular mycorrhiza (AM) represents an ancient endosymbiosis between plant roots and Glomeromycota fungi. Strigolactones (SLs), plant-derived terpenoid lactones, activate hyphal branching of AM fungi before physical contact. Lack of SL biosynthesis results in lower colonization of AM fungi.
- The F-box protein, DWARF3 (D3), and the hydrolase family protein DWARF14 (D14) are crucial for SL responses in rice. Here we conducted AM fungal colonization assays with the SL-insensitive d3 and d14 mutants.
- The d3 mutant exhibited strong defects in AM fungal colonization, whereas the d14 mutant showed higher AM fungal colonization. As D14 has a homologous protein, D14-LIKE, we generated D14-LIKE knockdown lines by RNA interference in the wildtype and d14 background. D14 and D14-LIKE double knockdown lines exhibited similar colonization rates as those of the d14-1 mutant.
- D3 is crucial for establishing AM symbiosis in rice, whereas D14 and D14-LIKE are not. Our results suggest distinct roles for these SL-related components in AM symbiosis.