These authors contributed equally to this work.
Intraspecific variation in mitochondrial genome sequence, structure, and gene content in Silene vulgaris, an angiosperm with pervasive cytoplasmic male sterility
Article first published online: 26 SEP 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 196, Issue 4, pages 1228–1239, December 2012
How to Cite
Sloan, D. B., Müller, K., McCauley, D. E., Taylor, D. R. and Štorchová, H. (2012), Intraspecific variation in mitochondrial genome sequence, structure, and gene content in Silene vulgaris, an angiosperm with pervasive cytoplasmic male sterility. New Phytologist, 196: 1228–1239. doi: 10.1111/j.1469-8137.2012.04340.x
- Issue published online: 5 NOV 2012
- Article first published online: 26 SEP 2012
- Manuscript Accepted: 17 AUG 2012
- Manuscript Received: 2 JUL 2012
- National Science Foundation. Grant Numbers: MCB-1022128, DEB-1050331
- the Czech Republic. Grant Number: GAČR 521/09/0261
- Ministry of Education, Youth and Sports of the Czech Republic. Grant Numbers: MŠMT LC06004, MŠMT Kontakt ME09035
- cytoplasmic male sterility (CMS);
- intracellular gene transfer;
- mitochondrial genome;
- Silene vulgaris
- In angiosperms, mitochondrial-encoded genes can cause cytoplasmic male sterility (CMS), resulting in the coexistence of female and hermaphroditic individuals (gynodioecy).
- We compared four complete mitochondrial genomes from the gynodioecious species Silene vulgaris and found unprecedented amounts of intraspecific diversity for plant mitochondrial DNA (mtDNA).
- Remarkably, only about half of overall sequence content is shared between any pair of genomes. The four mtDNAs range in size from 361 to 429 kb and differ in gene complement, with rpl5 and rps13 being intact in some genomes but absent or pseudogenized in others. The genomes exhibit essentially no conservation of synteny and are highly repetitive, with evidence of reciprocal recombination occurring even across short repeats (< 250 bp). Some mitochondrial genes exhibit atypically high degrees of nucleotide polymorphism, while others are invariant. The genomes also contain a variable number of small autonomously mapping chromosomes, which have only recently been identified in angiosperm mtDNA. Southern blot analysis of one of these chromosomes indicated a complex in vivo structure consisting of both monomeric circles and multimeric forms.
- We conclude that S. vulgaris harbors an unusually large degree of variation in mtDNA sequence and structure and discuss the extent to which this variation might be related to CMS.