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Fig. S1 Verification of knock-out (KO) mutant genotypes.

Fig. S2 Verification of genotypes of over-expressing lines.

Fig. S3 Nonphotochemical quenching (NPQ) kinetics of psbs and/or lhcsr knock-out (KO) lines.

Fig. S4 Comparison of PSBS-dependent nonphotochemical quenching (NPQ) in Physcomitrella patens and Arabidopsis.

Fig. S5 Carotenoid content in wild type (WT) and selected mutant/over-expresser (OE) lines.

Fig. S6 Light sensitivity of Physcomitrella patens lines.

Fig. S7 Correlation between nonphotochemical quenching (NPQ) values and PSBS/LHCSR accumulation.

Fig. S8 Scheme of evolution of nonphotochemical quenching (NPQ) mechanisms from algae to vascular plants.

Table S1. Primers employed for amplification and cloning of Physcomitrella PSBS or LHCSR1 coding sequences from cDNA