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Fig. S1 Complementation of the fah1×fah2 double mutant.

Fig. S2 Metabolite fingerprinting of fah1×fah2 mutant leaves confirms ceramides and glucosylceramides as mutation markers of the nonpolar extraction phase.

Fig. S3 Phytohormone analysis of the fah1×fah2 mutant leaves confirms salicylic acid (SA) and derivatives to be enriched in double-mutant plants.

Fig. S4 Comparison of the expression of different sphingolipid metabolism genes in different organs and developmental stages in Arabidopsis.

Fig. S5 Expression analysis of α-DOX1 and α-DOX2 in wild-type and mutant plants.

Table S1 Primers for genotyping of SALK/SAIL lines and reverse transcriptase-polymerase chain reaction (RT-PCR)

Table S2 Primers for complementation and promoter–β-glucuronidase (GUS) fusions

Table S3 Ceramide and glucosylceramides detected in the nonpolar extraction phase by the nontargeted metabolite fingerprinting approach

Table S4 Metabolite marker of fah1×fah2 detected in the polar extraction phase by the nontargeted metabolite fingerprinting approach (ultra-performance liquid chromatography-electrospray ionization-time of flight-mass spectrometry (UPLC-ESI-TOF-MS))

nph4351-sup-0002-TableS5.xlsxapplication/msexcel854KTable S5 Dataset of 2039 high-quality marker candidates (false discovery rate (FDR) < 0.01) from the nontargeted ultra-performance liquid chromatography-electrospray ionization-time of flight-mass spectrometry (UPLC-ESI-TOF-MS) analysis of the nonpolar extraction phase of wild-type and fah1×fah2 mutant leaves of Arabidopsis
nph4351-sup-0003-TableS6.xlsxapplication/msexcel790KTable S6 Dataset of 1649 high-quality marker candidates (false discovery rate (FDR) < 106) from the nontargeted ultra-performance liquid chromatography-electrospray ionization-time of flight-mass spectrometry (UPLC-ESI-TOF-MS) analysis of the polar extraction phase of wild-type and fah1×fah2 mutant leaves of Arabidopsis