An R2R3 MYB transcription factor in wheat, TaPIMP1, mediates host resistance to Bipolaris sorokiniana and drought stresses through regulation of defense- and stress-related genes
Article first published online: 9 OCT 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 196, Issue 4, pages 1155–1170, December 2012
How to Cite
Zhang, Z., Liu, X., Wang, X., Zhou, M., Zhou, X., Ye, X. and Wei, X. (2012), An R2R3 MYB transcription factor in wheat, TaPIMP1, mediates host resistance to Bipolaris sorokiniana and drought stresses through regulation of defense- and stress-related genes. New Phytologist, 196: 1155–1170. doi: 10.1111/j.1469-8137.2012.04353.x
- Issue published online: 5 NOV 2012
- Article first published online: 9 OCT 2012
- Manuscript Accepted: 22 AUG 2012
- Manuscript Received: 30 MAY 2012
- NSFC program. Grant Number: 30871523
- ‘863’ programs. Grant Numbers: 2012AA10A309, 2012AA101105
- Key Sci-Tech. Grant Number: 2013ZX08002001
- Bipolaris sorokiniana ;
- common wheat;
- MYB transcription factor;
- resistant response;
- Triticum aestivum
In this study, we report new insights into the function of a wheat (Triticum aestivum) MYB gene TaPIMP1 through overexpression and underexpression, and its underlying mechanism in wheat.
Electrophoretic mobility shift and yeast-one-hybrid assays indicated that TaPIMP1 can bind to five MYB-binding sites including ACI, and activate the expression of the genes with the cis-element, confirming that TaPIMP1 is an MYB transcription activator.
TaPIMP1-overexpressing transgenic wheat exhibited significantly enhanced resistance to the fungal pathogen Bipolaris sorokiniana and drought stresses, whereas TaPIMP1-underexpressing transgenic wheat showed more susceptibility to the stresses compared with untransformed wheat, revealing that TaPIMP1 positively modulates host-defense responses to B. sorokiniana and drought stresses. Microarray analysis showed that a subset of defense- and stress-related genes were up-regulated by TaPIMP1. These genes, including TaPIMP1, RD22, TLP4 and PR1a, were regulated by ABA and salicylic acid (SA). TaPIMP1-underexpressing transgenic wheat showed compromised induction of these stress-responsive genes following ABA and SA treatments.
In summary, TaPIMP1, as a positive molecular linker, mediates resistance to B. sorokiniana and drought stresses by regulation of stress-related genes in ABA- and SA-signaling pathways in wheat. Furthermore, TaPIMP1 may provide a transgenic tool for engineering multiple-resistance wheat in breeding programs.