An R2R3 MYB transcription factor in wheat, TaPIMP1, mediates host resistance to Bipolaris sorokiniana and drought stresses through regulation of defense- and stress-related genes

Authors

  • Zengyan Zhang,

    Corresponding author
    • The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops of Agricultural Ministry, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
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  • Xin Liu,

    1. The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops of Agricultural Ministry, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
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  • Xindong Wang,

    1. The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops of Agricultural Ministry, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
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  • Miaoping Zhou,

    1. Biotechnology Institute, Jiangsu Academy of Agricultural Sciences, Nanjing, China
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  • Xianyao Zhou,

    1. The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops of Agricultural Ministry, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
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  • Xingguo Ye,

    1. The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops of Agricultural Ministry, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
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  • Xuening Wei

    1. The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops of Agricultural Ministry, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
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Author for correspondence:

Zengyan Zhang

Tel: +86 10 82108781

Email: zhangzy@mail.caas.net.cn

Summary

  • In this study, we report new insights into the function of a wheat (Triticum aestivum) MYB gene TaPIMP1 through overexpression and underexpression, and its underlying mechanism in wheat.

  • Electrophoretic mobility shift and yeast-one-hybrid assays indicated that TaPIMP1 can bind to five MYB-binding sites including ACI, and activate the expression of the genes with the cis-element, confirming that TaPIMP1 is an MYB transcription activator.

  • TaPIMP1-overexpressing transgenic wheat exhibited significantly enhanced resistance to the fungal pathogen Bipolaris sorokiniana and drought stresses, whereas TaPIMP1-underexpressing transgenic wheat showed more susceptibility to the stresses compared with untransformed wheat, revealing that TaPIMP1 positively modulates host-defense responses to B. sorokiniana and drought stresses. Microarray analysis showed that a subset of defense- and stress-related genes were up-regulated by TaPIMP1. These genes, including TaPIMP1, RD22, TLP4 and PR1a, were regulated by ABA and salicylic acid (SA). TaPIMP1-underexpressing transgenic wheat showed compromised induction of these stress-responsive genes following ABA and SA treatments.

  • In summary, TaPIMP1, as a positive molecular linker, mediates resistance to B. sorokiniana and drought stresses by regulation of stress-related genes in ABA- and SA-signaling pathways in wheat. Furthermore, TaPIMP1 may provide a transgenic tool for engineering multiple-resistance wheat in breeding programs.

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