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Fig. S1 Phylogeny of TaPIMP1 was constructed based on peptide sequences using a neighbor–joining method of MEGA 4 software after these MYB protein sequences were aligned using ClustalW.

Fig. S2 The germination and growth of the stable TaPIMP1-overexpressing transgenic wheat lines (M80 and M499 in T3 generation) and wildtype Yangmai 12 (WT) plants as well as segregants lacking the TaPIMP1 transgene (Null).

Fig. S3 Microscopy images of leaf stomatal apertures of the wildtype and TaPIMP1-overexpressing transgenic plants withholding water for 0, 5, and 16 d.

Fig. S4 The transcript profiles of TaPIMP1 in the wildtype wheat Yangmai 12 were analyzed by qRT-PCR after treatment with 0.1 mM ABA, 1 mM SA, 0.1 mM JA, and 0.2 mM ET for 1, 3, 6, and 12 h.

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Table S1 Sequences of primers used in this study

Table S2 Promoter sequence analysis of stress-related genes up-regulated by TaPIMP1

Table S3 Major agronomic traits in TaPIMP1 transgenic and wildtype wheat plants


Table S4 Raw data of microarray analyses in the TaPIMP1-overexpressing and wildtype wheat lines

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Table S5 Genes up-regulated by TaPIMP1 through microarray analysis