Identification of novel virulence genes and metabolic pathways required for full fitness of Pseudomonas savastanoi pv. savastanoi in olive (Olea europaea) knots
Article first published online: 22 OCT 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 196, Issue 4, pages 1182–1196, December 2012
How to Cite
Matas, I. M., Lambertsen, L., Rodríguez-Moreno, L. and Ramos, C. (2012), Identification of novel virulence genes and metabolic pathways required for full fitness of Pseudomonas savastanoi pv. savastanoi in olive (Olea europaea) knots. New Phytologist, 196: 1182–1196. doi: 10.1111/j.1469-8137.2012.04357.x
- Issue published online: 5 NOV 2012
- Article first published online: 22 OCT 2012
- Manuscript Accepted: 17 AUG 2012
- Manuscript Received: 12 JUN 2012
- metabolic pathway;
- olive knot;
- Pseudomonas savastanoi ;
- Pseudomonas syringae ;
- signature-tagged mutagenesis;
- woody plant
- Comparative genomics and functional analysis of Pseudomonas syringae and related pathogens have mainly focused on diseases of herbaceous plants; however, there is a general lack of knowledge about the virulence and pathogenicity determinants required for infection of woody plants.
- Here, we applied signature-tagged mutagenesis (STM) to Pseudomonas savastanoi pv. savastanoi during colonization of olive (Olea europaea) knots, with the goal of identifying the range of genes linked to growth and symptom production in its plant host.
- A total of 58 different genes were identified, and most mutations resulted in hypovirulence in woody olive plants. Sequence analysis of STM mutations allowed us to identify metabolic pathways required for full fitness of P. savastanoi in olive and revealed novel mechanisms involved in the virulence of this pathogen, some of which are essential for full colonization of olive knots by the pathogen and for the lysis of host cells.
- This first application of STM to a P. syringae-like pathogen provides confirmation of functional capabilities long believed to play a role in the survival and virulence of this group of pathogens but not adequately tested before, and unravels novel factors not correlated previously with the virulence of other plant or animal bacterial pathogens.