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Fig. S1 Schematic representation of the signature-tagged mutagenesis (STM) screening strategy followed in this study for the selection of P. savastanoi pv. savastanoi mutants with reduced competitiveness in olive plants.

Fig. S2 DNA context of transposon insertions in P. savastanoi pv. savastanoi fitness-attenuated mutants (FAMs) probably affecting transcription of downstream genes.

Table S1 Plasmids constructed in this work

Table S2 Oligonucleotides used this work

Table S3 Number of siblings and independent insertions of the signature-tagged mutagenesis (STM) transposon in fitness-attenuated mutant (FAM) strains

Table S4 Enzymes involved in the different steps of the metabolic pathways required by P. savastanoi pv. savastanoi for full fitness in olive knots (Fig. 2)

Table S5 Pseudomonas savastanoi pv. savastanoi fitness-attenuated mutant (FAM) genes involved in processes previously identified by signature-tagged mutagenesis (STM) in human and animal bacterial pathogens.