These authors contributed equally to this work.
Transcription activator-like (TAL) effectors targeting OsSWEET genes enhance virulence on diverse rice (Oryza sativa) varieties when expressed individually in a TAL effector-deficient strain of Xanthomonas oryzae
Article first published online: 18 OCT 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 196, Issue 4, pages 1197–1207, December 2012
How to Cite
Verdier, V., Triplett, L. R., Hummel, A. W., Corral, R., Cernadas, R. A., Schmidt, C. L., Bogdanove, A. J. and Leach, J. E. (2012), Transcription activator-like (TAL) effectors targeting OsSWEET genes enhance virulence on diverse rice (Oryza sativa) varieties when expressed individually in a TAL effector-deficient strain of Xanthomonas oryzae. New Phytologist, 196: 1197–1207. doi: 10.1111/j.1469-8137.2012.04367.x
- Issue published online: 5 NOV 2012
- Article first published online: 18 OCT 2012
- Manuscript Accepted: 31 AUG 2012
- Manuscript Received: 3 MAY 2012
- Marie Curie IOF Fellowship. Grant Number: PIOF-GA-2009-235457
- CSU Infectious Diseases Supercluster
- the United States Agency for International Development Linkage Project
- Colorado Agricultural Experiment Station
- National Science Foundation. Grant Number: 0820831
- OsSWEET ;
- transcription activator-like (TAL) effectors;
- Xanthomonas oryzae
Genomes of the rice (Oryza sativa) xylem and mesophyll pathogens Xanthomonas oryzae pv. oryzae (Xoo) and pv. oryzicola (Xoc) encode numerous secreted transcription factors called transcription activator-like (TAL) effectors. In a few studied rice varieties, some of these contribute to virulence by activating corresponding host susceptibility genes. Some activate disease resistance genes. The roles of X. oryzae TAL effectors in diverse rice backgrounds, however, are poorly understood.
Xoo TAL effectors that promote infection by activating SWEET sucrose transporter genes were expressed in TAL effector-deficient X. oryzae strain X11-5A, and assessed in 21 rice varieties. Some were also tested in Xoc on variety Nipponbare. Several Xoc TAL effectors were tested in X11-5A on four rice varieties.
Xoo TAL effectors enhanced X11-5A virulence on most varieties, but to varying extents depending on the effector and variety. SWEET genes were activated in all tested varieties, but increased virulence did not correlate with activation level. SWEET activators also enhanced Xoc virulence on Nipponbare. Xoc TAL effectors did not alter X11-5A virulence.
SWEET-targeting TAL effectors contribute broadly and non-tissue-specifically to virulence in rice, and their function is affected by host differences besides target sequences. Further, the utility of X11-5A for characterizing individual TAL effectors in rice was established.