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nph4369-sup-0001-FiguresS1-S10_TableS1.pdfapplication/PDF1722K

Fig. S1 Sequence alignment of the N-termini of iron superoxide dismutases (FeSODs) from different species.

Fig. S2 Affinity purification of the glutathione-S-transferase (GST)-tagged FSD1, FSD2, FSD3 and CHAPERONIN 20 (CPN20).

Fig. S3 Active and inactive FSD1 in affinity-purified Holo-FSD1 and Apo-FSD1.

Fig. S4 Holo- and Apo-FSD1 activation by incubation with fsd1-desalted cellular extract.

Fig. S5 Sequence alignment of the CHAPERONIN 20 (CPN20) N- and C-domains and CPN10 among different species.

Fig. S6 Effect of Tris buffer, various pH buffers and metal ions on Holo-FSD1 activity.

Fig. S7 CHAPERONIN 20 (CPN20) facilitated Apo-FSD1 activation in vitro.

Fig. S8 The CHAPERONIN 60 (CPN60) antibody cross-reacted with all the chloroplast CPN60 subtypes and the affinity purified CPN20 without bacterial GroEL contamination.

Fig. S9 Phenotype of tomato wild-type (WT) and CHAPERONIN 20 (CPN20) virus-induced gene silenced plants.

Fig. S10 CSD1 activity and protein levels were not affected by the co-expression of CHAPERONIN 20 (CPN20) and mutants with defective co-chaperone activity.

Table S1 Primers used in this study

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Notes S1 LC-MS/MS analysis of high-performance liquid chromatography (HPLC) fractions.

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Notes S2 LC-MS/MS analysis of gel filtration fractions.