These authors contributed equally to this work.
CHAPERONIN 20 mediates iron superoxide dismutase (FeSOD) activity independent of its co-chaperonin role in Arabidopsis chloroplasts
Article first published online: 11 OCT 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 197, Issue 1, pages 99–110, January 2013
How to Cite
Kuo, W. Y., Huang, C. H., Liu, A. C., Cheng, C. P., Li, S. H., Chang, W. C., Weiss, C., Azem, A. and Jinn, T. L. (2013), CHAPERONIN 20 mediates iron superoxide dismutase (FeSOD) activity independent of its co-chaperonin role in Arabidopsis chloroplasts. New Phytologist, 197: 99–110. doi: 10.1111/j.1469-8137.2012.04369.x
- Issue published online: 26 NOV 2012
- Article first published online: 11 OCT 2012
- Manuscript Accepted: 3 SEP 2012
- Manuscript Received: 29 AUG 2012
- National Science Council, Taiwan. Grant Numbers: 98-2311-B-002-007-MY3, 101-2311-B-002-001
- NTU. Grant Number: 101R892003
- BARD. Grant Number: US-4443-11
Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing material) should be directed to the New Phytologist Central Office.
Fig. S1 Sequence alignment of the N-termini of iron superoxide dismutases (FeSODs) from different species.
Fig. S2 Affinity purification of the glutathione-S-transferase (GST)-tagged FSD1, FSD2, FSD3 and CHAPERONIN 20 (CPN20).
Fig. S3 Active and inactive FSD1 in affinity-purified Holo-FSD1 and Apo-FSD1.
Fig. S4 Holo- and Apo-FSD1 activation by incubation with fsd1-desalted cellular extract.
Fig. S5 Sequence alignment of the CHAPERONIN 20 (CPN20) N- and C-domains and CPN10 among different species.
Fig. S6 Effect of Tris buffer, various pH buffers and metal ions on Holo-FSD1 activity.
Fig. S7 CHAPERONIN 20 (CPN20) facilitated Apo-FSD1 activation in vitro.
Fig. S8 The CHAPERONIN 60 (CPN60) antibody cross-reacted with all the chloroplast CPN60 subtypes and the affinity purified CPN20 without bacterial GroEL contamination.
Fig. S9 Phenotype of tomato wild-type (WT) and CHAPERONIN 20 (CPN20) virus-induced gene silenced plants.
Fig. S10 CSD1 activity and protein levels were not affected by the co-expression of CHAPERONIN 20 (CPN20) and mutants with defective co-chaperone activity.
Table S1 Primers used in this study
Notes S1 LC-MS/MS analysis of high-performance liquid chromatography (HPLC) fractions.
Notes S2 LC-MS/MS analysis of gel filtration fractions.