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Summary

Factors influencing the spectrophotometric examination of liquor amnii have been investigated. Various methods of clarification and handling of liquor samples are compared, and their effects upon results obtained by seven currently used methods of assessment of bilirubin content are described. It is essential to follow the exact method originally described for each method of expression of liquor bilirubin content when using prediction graphs or tables to indicate severity of rhesus haemolytic disease.