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THE MAINTENANCE OF HUMAN AMNIOTIC MEMBRANES IN CULTURE
Article first published online: 23 AUG 2005
DOI: 10.1111/j.1471-0528.1981.tb00984.x
Issue
1471-0528/asset/cover.gif?v=1&s=b4e1d96c46e18c61210d584b63c13ee375cf562b "BJOG: An International Journal of Obstetrics & Gynaecology")
BJOG: An International Journal of Obstetrics & Gynaecology
Volume 88, Issue 3, pages 294–300, March 1981
Additional Information
How to Cite
Burgos, H. and Faulk, W. P. (1981), THE MAINTENANCE OF HUMAN AMNIOTIC MEMBRANES IN CULTURE. BJOG: An International Journal of Obstetrics & Gynaecology, 88: 294–300. doi: 10.1111/j.1471-0528.1981.tb00984.x
Publication History
- Issue published online: 23 AUG 2005
- Article first published online: 23 AUG 2005
- Received July 25, 1980/Accepted September 22, 1980
- Abstract
- References
- Cited By
Summary
A culture system is described for the long-term maintenance of human amnion in vitro. This has been developed because of the need for an amnion organ culture to study immunological aspects of this extra-embryonic membrane as well as the increasing surgical use of amnion in wound healing. The intact amnion can be maintained for 2–3 weeks with 50–90 per cent viability as determined by morphological and trypan-blue extraction techniques. This puts forward the feasability of developing an amnion bank for clinical investigations of wound healing as well as the possibility of in vitro studies of this membrane in the materno-fetal relationship in human pregnancy.