PLACENTAL PROTEIN 5 (PP5): DEVELOPMENT OF A RADIOIMMUNOASSAY AND MEASUREMENT OF CIRCULATING LEVELS IN NORMAL PREGNANCY

  1. A. D. Nisbet1,
  2. R. D. Bremner1,
  3. R. Herriot1,
  4. V. Jandial1,
  5. C. H. W. Horne1,
  6. H. Bohn2

Article first published online: 23 AUG 2005

DOI: 10.1111/j.1471-0528.1981.tb01021.x

Issue

BJOG: An International Journal of Obstetrics & Gynaecology

BJOG: An International Journal of Obstetrics & Gynaecology

Volume 88, Issue 5, pages 484–491, May 1981

Additional Information

How to Cite

Nisbet, A. D., Bremner, R. D., Herriot, R., Jandial, V., Horne, C. H. W. and Bohn, H. (1981), PLACENTAL PROTEIN 5 (PP5): DEVELOPMENT OF A RADIOIMMUNOASSAY AND MEASUREMENT OF CIRCULATING LEVELS IN NORMAL PREGNANCY. BJOG: An International Journal of Obstetrics & Gynaecology, 88: 484–491. doi: 10.1111/j.1471-0528.1981.tb01021.x

Author Information

  1. 1

    Department of Pathology, University Medical Buildings, Foresterhill, Aberdeen, and Department of Obstetrics and Gynaecology, Aberdeen Maternity Hospital Foresterhill, Aberdeen AB9 2ZD

  2. 2

    Behringwerke AG, Marburg/Lahn, West Germany

Publication History

  1. Issue published online: 23 AUG 2005
  2. Article first published online: 23 AUG 2005
  3. Received September 8, 1980/Accepted December 11, 1980

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Summary

A specific and sensitive, double antibody, solid phase radioimmunoassay was developed for measurement of placental protein 5 (PP5) in serum. A sensitivity limit of 0.8 μg/1 was achieved by pre-incubation with antiserum and use of a small assay volume. Single serum samples from 244 retrospectively proven normal pregnancies were used to construct a normal range of PP5 concentrations. PP5 was detectable at week 7 and rose to reach a maximum mean value of 54 μg/1 at weeks 36–37. Diurnal variation of PP5 concentration was similar to that encountered from day to day, and slightly greater than variation due to the radioimmunoassay. The decline of serum PP5 concentration post partum was initially rapid (half-life 5–14 minutes), but slowed after 0.5–1 hours; immunoreactive PP5 was still detectable seven days after delivery.

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