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Summary. Pregnancy-associated plasma protein A (PAPP-A) was measured throughout the menstrual cycle in eight women at daily intervals. The protein could be detected in all serum samples, and one patient showed consistently high PAPP-A values during the entire observation period. Twenty-six more women were tested by measuring a single serum specimen. No further high PAPP-A levels were discovered in these. There is no significant difference in PAPP-A concentration between the follicular and the luteal phase of the cycle.