• Agglutination;
  • contraceptive;
  • mouse;
  • Staphylococcus aureus

Objective  To investigate the contraceptive effect of sperm-agglutinating factor (SAF) isolated from Staphylococcus aureus in mouse.

Design  Experimental study performed with LACA mice obtained from the Central Animal House, Panjab University, Chandigarh, India.

Setting In vivo studies conducted in the Department of Microbiology, Panjab University, Chandigarh, India.

Population  Sixty female and 18 male mice were used for the studies.

Methods  Mice sperm–S. aureus agglutination, scanning electron microscopy (SEM), in vivo studies in mice.

Main outcome measure  Contraceptive efficacy of SAF.

Results S. aureus readily adhered to and agglutinated mouse spermatozoa. By SEM, S. aureus adherence was observed on sperm head as well as sperm tail. SAF was found to be causing 100% fertility inhibition in mouse with no effect on vaginal epithelium.

Conclusions  Sperm-agglutinating factor has been found to have a potential as a contraceptive agent.