The feasibility of a less invasive method to assess endometrial maturation—comparison of simultaneously obtained uterine secretion and tissue biopsy
Article first published online: 12 DEC 2008
© 2008 The Authors Journal compilation © RCOG 2008 BJOG An International Journal of Obstetrics and Gynaecology
BJOG: An International Journal of Obstetrics & Gynaecology
Special Issue: Emerging Technologies in Obstetrics and Gynaecology
Volume 116, Issue 2, pages 304–312, January 2009
How to Cite
van der Gaast, M., Macklon, N., Beier-Hellwig, K., Krusche, C., Fauser, B., Beier, H. and Classen-Linke, I. (2009), The feasibility of a less invasive method to assess endometrial maturation—comparison of simultaneously obtained uterine secretion and tissue biopsy. BJOG: An International Journal of Obstetrics & Gynaecology, 116: 304–312. doi: 10.1111/j.1471-0528.2008.02039.x
- Issue published online: 12 DEC 2008
- Article first published online: 12 DEC 2008
- Accepted 14 October 2008.
- glycodelin A;
- leukaemia inhibitory factor;
- uterine secretion
Objective To compare the assessment of endometrial maturation parameters in endometrial secretion samples obtained by a novel minimally invasive technique with those assessed in tissue biopsies.
Design Prospective study.
Setting University Hospital.
Population Healthy female volunteers attending a gynaecological outpatient clinic.
Methods Endometrial secretion fluid and tissue sampling 5 days after a spontaneous ovulation assessed with ultrasound.
Main outcome measures Progesterone (P) receptor, Ki-67 expression and the Noyes criteria were used to date endometrial biopsies. In the endometrial fluid samples, glycodelin A (GdA), leukaemia inhibitory factor (LIF) and P levels were analysed, and protein content and electrophoresis patterns were determined.
Results All data were correlated to estradiol (E2) and P serum concentrations. The dating according to histology and immunohistochemical staining patterns correlated significantly with GdA levels (r = 0.376, P =0.048) in endometrial fluid samples as well with serum levels of E2 (r = 0.568, P =0.001) and P (r = 0.408, P =0.023). No correlation was observed between tissue dating and LIF levels and protein content in endometrial fluid samples.
Conclusions The measurement of GdA in endometrial secretion samples may provide a less invasive method for assessing endometrial maturation in potential conception cycles without disrupting implantation.