Acknowledgements—We wish to thank the Council of Scientific and Industrial Research India for the grant of a Pool Officership to one of us (C. B.) which enabled the undertaking of this work. This work benefited from the helpful discussions with Dr. R. Mazumder and Dr. D. K. Biswas.
EFFECT OF PENTAMETHYLENE TETRAZOLE (METRAZOL) ON RAT BRAIN PHOSPHORYLASE IN VITRO
Article first published online: 4 OCT 2006
Journal of Neurochemistry
Volume 15, Issue 2, pages 107–113, February 1968
How to Cite
Biswas, C. and Talwar, G. Y. (1968), EFFECT OF PENTAMETHYLENE TETRAZOLE (METRAZOL) ON RAT BRAIN PHOSPHORYLASE IN VITRO. Journal of Neurochemistry, 15: 107–113. doi: 10.1111/j.1471-4159.1968.tb06181.x
- Issue published online: 4 OCT 2006
- Article first published online: 4 OCT 2006
- (Received 15 May 1967)
—1. The effect of pentamethylene tetrazole (PTZ) or metrazol on rat brain phosphorylase (E.C.184.108.40.206.) was studied in vitro. The inhibitory action of PTZ was dependent on its concentration, being most marked in about 4 mM concentration. The inhibitory effect could be reversed to a great extent by inclusion of 5’AMP in the preincubation mixture. GMP, UMP and pyridoxal phosphate had no protective action under similar conditions.
2. PTZ did not appear to be a general inhibitor of enzymes. It had no effect on the activity of rat brain guanine deaminase, E. coli RNA-polymerase, or E. coli aspartate kinase. The inhibition of rat liver phosphorylase activity by PTZ was of a lower order (20 per cent) in comparison with that of brain phosphorylase (56 per cent).
3. Megimide, another convulsant drug, also inhibited the brain phosphorylase in vitro, but the effect of Nikethamide was not so pronounced. Pentothal sodium (a sedative drug) had very little effect on the brain phosphorylase activity.