Acknowledgements—The expert technical assistance of Sally Paxman, Cretyl Mills and Dorothy Farrar in various phases of this work is gratefully acknowledged.
EFFECT OF ION REMOVAL ON THE SOLUBILITY OF RAT BRAIN PROTEINS IN CHLOROFORM-METHANOL MIXTURES*
Article first published online: 4 OCT 2006
Journal of Neurochemistry
Volume 15, Issue 3, pages 153–159, March 1968
How to Cite
Lees†, M. B. (1968), EFFECT OF ION REMOVAL ON THE SOLUBILITY OF RAT BRAIN PROTEINS IN CHLOROFORM-METHANOL MIXTURES. Journal of Neurochemistry, 15: 153–159. doi: 10.1111/j.1471-4159.1968.tb06190.x
McLean Hospital Research Laboratory, Belmont, Mass. U.S.A.
- Issue published online: 4 OCT 2006
- Article first published online: 4 OCT 2006
- (Received 10 July 1967)
—The amount of chloroform-methanol-soluble protein obtained from rat brain tissue homogenates which have been subjected to washing by repeated contrifugation or dialysis is a several-fold greater than that obtained from untreated homogenates. The increase consequence of the removal of loosely bound electrolytes during the process of centrifugation or dialysis: little or no increase is observed upon (a) the addition of the supernatant, the ashed supernatant, or the diffusate to the washed homogenate; (b) the addition of inorganic salts to the washed homogenate; or (c) washing of the homogenate with a salt solution.
The previously observed effect of sucrose in increasing the amount of chloroform–methanol-soluble protein obtained from subcellular fractions is apparently superimposed upon the effect of the removal of salts.