INFLUENCE OF AGE IN THE INCORPORATION OF [14C]GLYCINE INTO ISOLATED RAT NERVE SEGMENTS IN VITRO

Authors


  • Acknowledgements—It is a pleasure to acknowledge my indebtedness to Professor J. B. Cavanagh, not only for initiating the project, but also for his constant help and encouragement throughout the many stages of its development. This work was supported by the Medical Research Council.

Abstract

—(1) Sciatic nerves of young rats have been shown to incorporate [14C]glycine in vitro into the protein fraction at a higher rate than nerves from adult rats under the same conditions.

(2) Whilst there is little change in the DNA content of the sciatic nerve of rats with age, there is a is noticeable decline in the RNA content with age.

(3) There a linear relationship between the specific activity of the protein fraction and the RNA content of the sciatic nerve under in vitro conditions.

(4) There is a decline in the specific activity of the protein fraction with age when expressed against DNA.

(5) A linear relationship exists between the logarithm of the specific activity and the length of the femur.

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