Acknowledgements—Our thanks are due to Dr. G. M. Gray for reference lipid compounds and to Dr. W. G. P. Mair for the electron microphotography.
SPHINGOLIPIDS AND PHOSPHOLIPIDS IN MICROSOMES AND MYELIN FROM NORMAL AND PATHOLOGICAL BRAINS
Article first published online: 4 OCT 2006
Journal of Neurochemistry
Volume 15, Issue 3, pages 243–248, March 1968
How to Cite
Cumings, J. N., Thompson, E. J. and Goodwin, H. (1968), SPHINGOLIPIDS AND PHOSPHOLIPIDS IN MICROSOMES AND MYELIN FROM NORMAL AND PATHOLOGICAL BRAINS. Journal of Neurochemistry, 15: 243–248. doi: 10.1111/j.1471-4159.1968.tb06203.x
- Issue published online: 4 OCT 2006
- Article first published online: 4 OCT 2006
- (Received 27 September/i 1967)
—Myelin and microsomes were separated from human cerebral white matter and cortex respectively using the technique of 15% caesium chloride and their sphingolipid and phospholipid contents estimated. Normal brains as well as cerebral material from cases of metachromatic leucodystrophy, Krabbe's disease and Tay-Sachs’disease were studied.
Gangliosides were not present in normal myelin but were found in microsomes and in myelin from the pathological material. The ratio of cerebroside to sulphatide in myelin was 4 to 1 in normal, 1 to 20 in metachromatic and 7 to 1 in Krabbe's disease.
The results in the human material are briefly discussed.