ISOLATION AND CHARACTERIZATION OF GLYCOSAMINOGLYCANS IN HUMAN BRAIN OF DIFFERENT AGE GROUPS

Authors


  • Acknowledgements—The work was supported by Grant No. NIH-IND-6X4324 from the National Institute of Health, U.S. Public Health Service, and a grant from the Council of Scientific and Industrial Research, India. The excellent technical assistance of Mr. Roy Cherian is gratefully acknowledged. The keen interest of Principal J. Chandy of this institute as well as the kind gesture of Dr. R. U. Margolis, Department of Pharmacology, New York University School of Medicine, New York, for making his manuscript available before its publication are deeply appreciated.

Abstract

—Five distinct glycosaminoglycan fractions have been isolated from human brain of various age groups, by employing an improved fractionation procedure. Analysis of these fractions showed that human brain contains hyaluronic acid, chondroitin-4-sulphate, chondroitin-6-sulphate, dermatan sulphate, heparan sulphate and two unidentified low sulphated fractions. The pattern of variation of these compounds with age, indicates that they may be playing an important role in the process of myelination and brain maturation.

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