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Abstract The sterol ester fraction in the human glioblastoma was isolated and characterized. Cholesterol was identified as the constituent sterol. Contained in the complex mixture of fatty acids were members with 0-4 double bonds and 14-28 carbon atoms. About 1/4 of the acids were polyunsaturated. Long chain members (C20 and above) composed 1/3 of the mixture. The results suggest that the sterol ester component in the glioblastoma does not derive from blood plasma.