Dr. Ho is Research Associate at Baylor College of Medicine, Houston, Texas. Reprint requests should be sent to Dr. Ellman.
TRITON SOLUBILIZED ACETYLCHOLINESTERASE OF BRAIN
Version of Record online: 4 OCT 2006
Journal of Neurochemistry
Volume 16, Issue 11, pages 1505–1513, November 1969
How to Cite
Ho, I. K. and Ellman, G. L. (1969), TRITON SOLUBILIZED ACETYLCHOLINESTERASE OF BRAIN. Journal of Neurochemistry, 16: 1505–1513. doi: 10.1111/j.1471-4159.1969.tb09905.x
- Issue online: 4 OCT 2006
- Version of Record online: 4 OCT 2006
- (Received 6 January 1969. Accepted 3 May 1969)
—The total AChE of brain can be readily extracted into aqueous Triton X-100. By column chromatography of these extracts a preparation was obtained at least six times as active as the original material and in yields of 60-80 per cent of the original amounts. The molecular weight of this material was estimated to be over 200,000. When brain tissue was treated with venom or bacterial protease, a water-soluble AChE was obtained with an overall purification of 150-fold. The most active preparation of AChE hydrolysed 880 μ-moles of acetylthiocholine/hr/mg of protein. The molecular weight of this preparation was estimated to be about 100,000. The enzyme which was extracted by Triton X-100 is probably the precursor of the more water-soluble enzyme that can be prepared from brain tissue by treatment with venom or protease.