CONTENT OF ATP IN CULTIVATED NEURONS AND ASTROCYTES EXPOSED TO BALANCED AND POTASSIUM-RICH MEDIA

Authors


  • The work was supported by grants from the Danish State Research Council (grant 511–75/69 and 512-57/69), the P. Carl Petersen Foundation and the Carlsberg Foundation. A travel grant to John Booher from the Pasadena Foundation for Medical Research, Pasadena, California, USA is cordially acknowledged.

Abstract

Abstract— The content of ATP was measured in neurons from dorsal root ganglia and in cortical and spinal astrocytes with the luciferase method. No ATP could be demonstrated in the cortical cells but in the dorsal root neurons and spinal astrocytes an average amount of 7·9 and 11·9 × 10−15 mol per cell respectively was found. An increase in the external potassium concentration had no effect on the content of ATP in neurons (7·1 × 10−15 mol/cell) but led to a significant (P < 0·005) decline to 6·4 × 10−15 mol per cell in the astrocytes.

Ancillary