Supported by Grants MH-12773, K3-MH-18232, NB-02255 and GM-1191.
COLCHICINE-BINDING ACTIVITY IN PARTICULATE FRACTIONS OF MOUSE BRAIN1
Article first published online: 4 OCT 2006
Journal of Neurochemistry
Volume 17, Issue 9, pages 1355–1364, September 1970
How to Cite
Feit, H. and Barondes, S. H. (1970), COLCHICINE-BINDING ACTIVITY IN PARTICULATE FRACTIONS OF MOUSE BRAIN. Journal of Neurochemistry, 17: 1355–1364. doi: 10.1111/j.1471-4159.1970.tb06870.x
- Issue published online: 4 OCT 2006
- Article first published online: 4 OCT 2006
- Received 24 December 1969. Accepted 12 March 1970
Both particulate and soluble fractions of brain homogenates bound [3H]colchicine. Approximately one-half of the total colchicine-binding activity in mouse brain was found in the particulate fraction. Of the particulate fractions, the microsomal and nerveending subfractions which sediment at the 1·0–1·2 m interface on sucrose gradients were richest in colchicine-binding activity. Intact microtubules were not found in these fractions, but colchicine-binding activity of these fractions may be related to the presence of microtubular protein.