This work is taken from the thesis which was submitted to University of Tokyo as the requirements for the Ph.D. degree.
SYNTHESIS AND RELEASE OF [14C]ACETYLCH0LINE IN SYNAPTOSOMES
Article first published online: 12 DEC 2006
Journal of Neurochemistry
Volume 18, Issue 6, pages 781–798, June 1971
How to Cite
Haga, T. (1971), SYNTHESIS AND RELEASE OF [14C]ACETYLCH0LINE IN SYNAPTOSOMES. Journal of Neurochemistry, 18: 781–798. doi: 10.1111/j.1471-4159.1971.tb12008.x
- Issue published online: 12 DEC 2006
- Article first published online: 12 DEC 2006
- (Received 22 June 1970. Accepted 25 August 1970)
Abstract— Synaptosomes took up [14C]choline, about half or more of which was converted to [I4C]acetylcholine when incubated in an appropriate medium containing 1 to 5 μM-[14C] choline and neostigmine. The amount of [14C]acetylcholine synthesized in synaptosomes increased in parallel with the increase of Na+ concentration in the incubation medium. The effect of Na+ on the uptake of [I4C]choline into synaptosomes was dependent on the concentration of choline in the incubation medium.
About 25 per cent of [14C]acetylcholine synthesized in synaptosomes was released rapidly into the medium by increasing the K+ concentration in the medium from 5 mm to 35 mm. The change of Na+ concentration hardly affected the release of [14C]acetylcholine. The effect of K+ on the release of [14C]choline was rather small compared to that on [14C] acetylcholine. Ouabain promoted the release of [14C]acetylcholine.