Postdoctoral Fellow in the laboratory of Dr. Morell, supported by Training Grant MH-06918 from the National Institute of Mental Health, U.S. Public Health Service. Present address: Institute of Medical Biochemistry, University of Aarhus, DK-8000 C, Aarhus, Denmark.
PROTEIN COMPOSITION OF MYELIN OF THE PERIPHERAL NERVOUS SYSTEM
Article first published online: 4 OCT 2006
Journal of Neurochemistry
Volume 20, Issue 4, pages 1207–1216, April 1973
How to Cite
Greenfield, S., Brostoff, S., Eylar, E. H. and Morell, P. (1973), PROTEIN COMPOSITION OF MYELIN OF THE PERIPHERAL NERVOUS SYSTEM. Journal of Neurochemistry, 20: 1207–1216. doi: 10.1111/j.1471-4159.1973.tb00089.x
- Issue published online: 4 OCT 2006
- Article first published online: 4 OCT 2006
- Received 29 August 1972. Accepted 17 November 1972
Abstract— Myelin was purified from the peripheral nervous system (PNS) of several species. The protein composition of these preparations was examined by discontinuous polyacrylamide gel electrophoresis in buffers containing sodium lauryl sulphate. Proteins characteristic of all samples include, in order of increasing mobility: a series of high molecular weight proteins, the major peripheral nerve protein (P0), two uncharacterized proteins, and two basic proteins (P1 and P2). Quantitative results, obtained by densitometry of gels stained with Fast Green showed differences in protein distribution, both between species, and from different types of nerves obtained from the same animal. The relative amounts of P1 and P2 proteins were the most variable; e.g. myelin from guinea-pig sciatic nerve had little or no P2 protein, whereas 15 per cent of the myelin protein of beef posterior intradural root was Pz protein. P0, P1 and P2 proteins from rabbit sciatic nerve and P0 and P2 proteins from beef dorsal and ventral intradural roots were purified and their amino acid compositions were determined. Our results indicated that the P1 protein is very similar in size and amino acid composition to the basic protein of central nervous system myelin, whereas the P0 and P2 proteins are unique to the PNS.