Present address: Department of Chemical Pathology, The Welsh National School of Medicine, Heath Park, Cardiff CF4 4XN.
STUDIES ON RNA SYNTHESIS IN TWO POPULATIONS OF NUCLEI FROM THE MAMMALIAN CEREBRAL CORTEX
Article first published online: 4 OCT 2006
Journal of Neurochemistry
Volume 21, Issue 1, pages 19–40, July 1973
How to Cite
THOMPSON, R. J. (1973), STUDIES ON RNA SYNTHESIS IN TWO POPULATIONS OF NUCLEI FROM THE MAMMALIAN CEREBRAL CORTEX. Journal of Neurochemistry, 21: 19–40. doi: 10.1111/j.1471-4159.1973.tb04222.x
- Issue published online: 4 OCT 2006
- Article first published online: 4 OCT 2006
- Received 23 January 1973. Accepted 7 March 1973)
A method is presented for the rapid separation of cell nuclei from the rabbit cerebral cortex into two populations. The first of these consists largely of nuclei with the morphological characteristics of neuronal nuclei, the second almost entirely of nuclei with the morphological characteristics of glial cell nuclei. From studies based upon sensitivity to the toxin α-amanitin, the ratio of incorporation of different bases, ionic requirements and differential sensitivity to actinomycin D, it is concluded that under both the classical low and high salt conditions described by other workers, two enzymes are active in RNA synthesis. The presence of a third enzyme of low activity cannot be excluded. No qualitative difference in the number of enzymes involved in RNA synthesis in neuronal and glial cell nuclei has been found, but there are quantitative differences in activity between the two nuclear populations.