1471-4159/asset/olbannerleft.gif?v=1&s=4636ca86ad7e40d133e71d09a5d759010472e0c2)
1471-4159/asset/olbannerright.gif?v=1&s=38094197c2d87aeb3b86aa293b5858c5c15cca29)
ENZYMATIC ACYLATION OF 1-ALKYL-, 1-ALKENYL-AND 1-ACYL GLYCERO-3-PHOSPHORYLETHANOLAMINE IN DEVELOPING RAT BRAIN
Article first published online: 4 OCT 2006
DOI: 10.1111/j.1471-4159.1974.tb06933.x
1471-4159/asset/cover.gif?v=1&s=4f99ec10d4bac18b4327882ae87f581a11d8dd49 "Journal of Neurochemistry")
Additional Information
How to Cite
Natarajan, V. and Sastry, P. S. (1974), ENZYMATIC ACYLATION OF 1-ALKYL-, 1-ALKENYL-AND 1-ACYL GLYCERO-3-PHOSPHORYLETHANOLAMINE IN DEVELOPING RAT BRAIN. Journal of Neurochemistry, 23: 187–192. doi: 10.1111/j.1471-4159.1974.tb06933.x
Publication History
- Issue published online: 4 OCT 2006
- Article first published online: 4 OCT 2006
- Received 26 November 1973. Accepted 11 January 1974
- Abstract
- Article
- References
- Cited By
Abstract— Rat brain particulate fractions were shown to acylate [32P]1-alkyl-sn-glycero-3-phosphorylethanolamine (GPE). While the main product is 1-alkyl-2-acyl GPE, about 12 per cent of the radioactivity was also found in 1-alkenyl-2-acyl GPE. The acyl transferase activity was completely dependent on added ATP and CoA and it was localized mainly in the microsomal fraction. A comparative study of acyl transferase activities to 1-alkyl-, 1-alkenyl-, and 1-acyl GPE by crude mitochondrial fraction and microsomes of 10, 16 and 22-day-old rat brains showed a progressive increase in activity with development. In the 22-day-old rat brain the order of activity towards the three substrates is as follows: 1-acyl GPE ± 1-alkenyl GPE ± 1-alkyl GPE with a crude mitochondrial fraction and 1-acyl GPE ± 1-alkyl GPE ± 1-alkenyl GPE with microsomes.