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EFFECT OF NEOMYCIN ON PHOSPHOINOSITIDE LABELLING AND CALCIUM BINDING IN GUINEA-PIG INNER EAR TISSUES IN VIVO AND IN VITRO

Authors

  • Angela Orsulakova,

    1. Kresge Hearing Research Institute and Department of Biological Chemistry. University of Michigan. Ann Arbor, MI 48104, U.S.A.
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    • 2

      A preliminary report has been published (Schacht, 1974a). This research was supported by NIH-NIEHS con tract No. NO1-ES-2-2110 and NIH Program Project Grant No. 05785.

  • Eva Stockhorst,

    1. Kresge Hearing Research Institute and Department of Biological Chemistry. University of Michigan. Ann Arbor, MI 48104, U.S.A.
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  • Jochen Schacht

    1. Kresge Hearing Research Institute and Department of Biological Chemistry. University of Michigan. Ann Arbor, MI 48104, U.S.A.
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  • Forschungslabor für Akustische Medizin, Universität Düsseldorf, 4 Düsseldorf 1, Germany.

Abstract

—Phospholipids of guinea-pig inner ear tissues were labelled in vivo by perilymphatic perfusion of the cochlea with [32P]orthophosphate or myo-[3H]inositol. After 20-40 min the most highly labelled 32P-lipids were phosphatidylinositol phosphate and diphosphate. Incorporation of [3H]inositol proceeded in the order phosphatidylinositol > phosphatidylinositol phosphate > phosphatidylinositol diphosphate.

After treatment of animals with neomycin for 3 weeks, 32P-incorporation into phosphatidylinositol diphosphate, but not into other lipids, was significantly decreased in the preparations of the organ of Corti and stria vascularis. In homogenates of inner ear tissues, the labelling of the polyphosphoinositides by [γ-32P]ATP was increased and the hydrolysis of these lipids was blocked in the presence of 10−4m-neomycin. Neomycin also competitively inhibited the binding of 45Ca2+ to homogenates of these tissues.

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