Department of Biochemistry, University of Connecticut Health Center, Farmington, CT.
ACCUMULATION OF THE FOUR MYELIN BASIC PROTEINS IN MOUSE BRAIN DURING DEVELOPMENT1
Article first published online: 5 OCT 2006
Journal of Neurochemistry
Volume 31, Issue 4, pages 779–782, October 1978
How to Cite
Barbarese, E., Carson, J. H. and Braun, P. E. (1978), ACCUMULATION OF THE FOUR MYELIN BASIC PROTEINS IN MOUSE BRAIN DURING DEVELOPMENT. Journal of Neurochemistry, 31: 779–782. doi: 10.1111/j.1471-4159.1978.tb00110.x
Research supported by the Medical Research Council of Canada and the Canadian Multiple Sclerosis Society.
- Issue published online: 5 OCT 2006
- Article first published online: 5 OCT 2006
- (Received 3 February 1978. Accepted 28 April 1978)
Myelin was isolated from the brains of mice 15, 20, 30, and 60 days after birth. The total amount of basic protein present in the isolated myelin was determined by radioimmunoassay. The 4 myelin basic proteins, with molecular weights of 21,500, 18,500, 17,000 and 14,000, were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and their relative amounts were determined densitometrically. The absolute amount of each of the basic proteins was calculated from its relative amount on the gel and from the total amount of myelin basic protein in the sample as determined by radioimmunoassay. The results show that between 10 and 30 days after birth each protein accumulates at a characteristic rate so that the molar ratios among the 4 basic proteins are (in descending order according to their molecular weights) 1:5:2:10 during this period. Between 30 and 60 days after birth the 14 K and 18.5 K proteins continue to accumulate at reduced rates while the 21.5 K and 17 K proteins begin to disappear from the myelin membrane; 60 days after birth the molar ratios among the 4 basic proteins are 1:10:3.5:35. These developmental patterns of accumulation are discussed in relation to the possible role of each of the 4 myelin basic proteins in myelination.