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STIMULATORY EFFECT OF l-GLUTAMATE AND RELATED AMINO ACIDS ON [3H]DOPAMINE RELEASE FROM RAT STRIATUM: AN IN VITRO MODEL FOR GLUTAMATE ACTIONS

Authors

  • P. J. Roberts,

    Corresponding author
    1. Department of Physiology and Pharmacology, School of Biochemical and Physiological Sciences, University of Southampton, Southampton SO9 3TU, U.K.
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  • Susan D. Anderson

    1. Department of Physiology and Pharmacology, School of Biochemical and Physiological Sciences, University of Southampton, Southampton SO9 3TU, U.K.
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Abstract

Abstract— The effects of l-glutamate and a number of structural analogues on the spontaneous release of [3H]dopamine from slices of rat striatum were examined. Glutamate, and other excitatory amino acids produced a marked stimulation of [3H]DA release which was Ca2+-dependent and unaffected by either procaine or tetrodotoxin. The glutamate-stimulated release was abolished in kainate-lesioned striatum. The action of glutamate was effectively antagonised by glutamamate diethylester and 2-amino-4-phosphonobutyric acid, but only weakly by l-methionine-dl-sulfoximine. Other proposed amino acid antagonists were inactive. The likely site of the releasing action of l-glutamate on presynaptic sites on nigro-striatal DA terminals is discussed.

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