Synthesis of Retinal Gangliosides During Chick Embryonic Development
Version of Record online: 12 DEC 2006
Journal of Neurochemistry
Volume 35, Issue 1, pages 100–108, July 1980
How to Cite
Panzetta, P., Macdoni, H. J. F. and Caputto, R. (1980), Synthesis of Retinal Gangliosides During Chick Embryonic Development. Journal of Neurochemistry, 35: 100–108. doi: 10.1111/j.1471-4159.1980.tb12494.x
- Issue online: 12 DEC 2006
- Version of Record online: 12 DEC 2006
- Received October 3, 1979; accepted January 7, 1980.
Abstract: Embryonic retina cells incorporated radioactivity from D-[6-3H]glucosamine into gangliosides in vitro. The incorporation was higher in retinas from younger embryos. The pattern of labeling of individual gangliosides of the retina changed gradually from a predominant labeling of gangliosides running chromatographically as GD3 (nomenclature of Svennerholm) and GM3 in retinas from 8-day-old embryos to a predominant labeling of those running as GDIa and GT, in retinas from 13–18-day-old embryos and newly hatched chicks. The shift in the pattern of labeling correlated with a temporary increase of about sixfold of the activity of UDP-N-acetyl-galactosamine:GM3 N-acetylgalactosaminyltransferase occurring between days 8 and 14 of embryonic development and with a regular increase of the activity of the UDP-galactose:GM2 galactosyltransferase occurring from day 8 until hatching. The activities of the CMP-NeuAc:lactosylceramide-, CMP-NeuAc:GM3-, and CMP-NeuAc:GM1-sialosyltransferases in the retinas of newly hatched chicks were 40, 20, and 40%(in comparison with the corresponding activities determined in retinas of the 8-day-old embryo.