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Synthesis of Retinal Gangliosides During Chick Embryonic Development

Authors

  • P. Panzetta,

    1. Departaniento de Quimica Bioloögicai, Facultad de Ciencrys Qnimicas, Universidad National de Cordoba, CÖrdoba 5000, Repú Argentina
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  • H. J. F. Macdoni,

    1. Departaniento de Quimica Bioloögicai, Facultad de Ciencrys Qnimicas, Universidad National de Cordoba, CÖrdoba 5000, Repú Argentina
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  • R. Caputto

    Corresponding author
    1. Departaniento de Quimica Bioloögicai, Facultad de Ciencrys Qnimicas, Universidad National de Cordoba, CÖrdoba 5000, Repú Argentina
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Address correspondence to Dr. Ranwel Caputto. Ganglioside species are named according to Svennerholm (1963).

Abstract

Abstract: Embryonic retina cells incorporated radioactivity from D-[6-3H]glucosamine into gangliosides in vitro. The incorporation was higher in retinas from younger embryos. The pattern of labeling of individual gangliosides of the retina changed gradually from a predominant labeling of gangliosides running chromatographically as GD3 (nomenclature of Svennerholm) and GM3 in retinas from 8-day-old embryos to a predominant labeling of those running as GDIa and GT, in retinas from 13–18-day-old embryos and newly hatched chicks. The shift in the pattern of labeling correlated with a temporary increase of about sixfold of the activity of UDP-N-acetyl-galactosamine:GM3 N-acetylgalactosaminyltransferase occurring between days 8 and 14 of embryonic development and with a regular increase of the activity of the UDP-galactose:GM2 galactosyltransferase occurring from day 8 until hatching. The activities of the CMP-NeuAc:lactosylceramide-, CMP-NeuAc:GM3-, and CMP-NeuAc:GM1-sialosyltransferases in the retinas of newly hatched chicks were 40, 20, and 40%(in comparison with the corresponding activities determined in retinas of the 8-day-old embryo.

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