This study was supported by grant BNS 05826 from the National Science Foundation and by the Kansas Agricultural Experiment Station. This is contribution 81-57-j, Division of Biology, the Kansas Agricultural Experiment Station, Kansas State University.
Effect of α-Methylphenylalanine and Phenylalanine on Brain Polyribosomes and Protein Synthesis
Article first published online: 5 OCT 2006
Journal of Neurochemistry
Volume 36, Issue 4, pages 1476–1484, April 1981
How to Cite
Binek, P. A., Johnson, T. C. and Kelly, C. J. (1981), Effect of α-Methylphenylalanine and Phenylalanine on Brain Polyribosomes and Protein Synthesis. Journal of Neurochemistry, 36: 1476–1484. doi: 10.1111/j.1471-4159.1981.tb00589.x
- Issue published online: 5 OCT 2006
- Article first published online: 5 OCT 2006
- Received September 2, 1980; accepted October 27, 1980.
- Protein synthesis
Abstract: A chronic hyperphenylalanemia was effectively produced in developing mice by daily administrations of phenylalanine (2 mg/g body wt) and a phenylalanine hydroxylase inhibitor α-methyl-D, L-phenylalanine (0.43 mg/g body wt). The presence of α-methylphenylalanine in newborn mice inhibited 65–70% of hepatic phenylalanine hydroxylase activity within 12 h. Since this maximum inhibition persisted for 24 h or longer, decreased enzyme activity was maintained by daily administrations. Whereas concentrations of phenylalanine increased approximately 40-fold in both plasma and brain following injection of α-methylphenylalanine and phenylalanine, plasma levels of tyrosine were not altered significantly. Concomitant with changes in phenylalanine concentrations we observed the brain polyribosomes' disaggregation, which reached a maximum 3 h after injection and persisted as long as 18 h. Polyribosomes did not become refractory to as many as 10 daily injections of α-methylphenylalanine and phenylalanine. In addition to polyribosome disaggregation, chronic hyperphenylalanemia reduced the rates of polypeptide chain elongation on polyribosomes isolated from brain homogenates.