Activity and Isoenzyme Pattern of Lactate Dehydrogenase in Neurons and Astroblasts Cultured from Brains of Chick Embryos

Authors

  • G. Tholey,

    1. Laboratoire de Biochimie Microbienne, Faculté de Pharmacie, Université Louis Pasteur, Strasbourg, France
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  • B. F. Roth-Schechter,

    Corresponding author
    1. Laboratoire de. Pharmacodynamie, Faculté de Pharmacie, Université Louis Pasteur, Strasbourg, France
      Address reprint requests and correspondence to Dr. B. F. Roth-Schechter, Laboratoire de Pharmacodynamie, P.O. 10, 67400 Strasbourg Cedex, France.
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  • P. Mandel

    1. Centre de Neurochime du CNRS, 11, rue Humann, 67085 Strasbourg Cedex, France
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  • The expert technical assistance of Mrs. M. A. Laluet and Miss G. Schmidt is gratefully acknowledged. This research was supported by grant INSERM-ATP 81-79-113 and grant INSERM-ATP 6-74-27.

Address reprint requests and correspondence to Dr. B. F. Roth-Schechter, Laboratoire de Pharmacodynamie, P.O. 10, 67400 Strasbourg Cedex, France.

Abstract:

Primary cultures of neurons and glial cells (astroblasts) prepared from brains of 8-day-old and 15-day-old chick embryos, respectively, were grown for periods between 3 and 19 days. Specific activity of lactate dehydrogenase (LDH) increased in both types of cultures as a function of time and was always significantly higher in glial cells than in neurons. Glial cell extracts were found to contain predominantly the anaerobic isoenzymatic form of LDH (LDH-H4), and this pattern did not change over a period of 19 days. Cultured neurons contained predominantly the aerobic isoenzymatic form LDH-H4, and there was a progressive appearance of all other isoenzymes over an 8-day period. These results support the hypothesis of a different energy metabolism in neurons and glia.

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