Identification and Characterization of Sulfhydryl-Containing Proteolytic Fragments Involved in the Ca2+-Induced Conformational Change of Beef Brain S-100

Authors


Address correspondence to Dr. K. G. Haglid, Institute of Neurobiology, P.O.B. 33 031, S-400 33 Göteborg, Sweden.

Address reprint requests to Dr. H. Nika, Department of Cell Physiology, Wenner-Gren Institute, University of Stockholm, Sweden.

Abstract

Abstract: The Ca2+-dependent conformational alteration of the brain-specific S-100 protein was studied by reacting the protein with N-ethyl[2,3-14C]maleimide in the absence and presence of Ca2+ and under denaturing conditions. Peptic hydrolysates of the 14C-labeled protein were analyzed and fractionated by high-performance liquid chromatography. Labeled peptide fractions were characterized by high-voltage electrophoresis and TLC. A clear distinction could be made between two classes of sulfhydryl-containing fragments: (a) neutral, hydrophobic, and (b) acidic. Ca2+ markedly favored 14C incorporation into the former components, whereas the latter were readily available only under denaturing conditions.

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