In Vivo Measurement of Dopamine and Its Metabolites by Intracerebral Dialysis: Changes After d-Amphetamine
Article first published online: 5 OCT 2006
Journal of Neurochemistry
Volume 41, Issue 6, pages 1769–1773, June 1983
How to Cite
Zetterström, T., Sharp, T., Marsden, C. A. and Ungerstedt, U. (1983), In Vivo Measurement of Dopamine and Its Metabolites by Intracerebral Dialysis: Changes After d-Amphetamine. Journal of Neurochemistry, 41: 1769–1773. doi: 10.1111/j.1471-4159.1983.tb00893.x
- Issue published online: 5 OCT 2006
- Article first published online: 5 OCT 2006
- Received February 28, 1983; revised May 21, 1983; accepted June 20, 1983.
- In vivo release;
- Brain dialysis;
- Ascorbic acid;
Abstract: By using a new technique, intracerebral dialysis, in combination with high performance liquid chromatography and electrochemical detection, it was possible to recover and measure endogenous extracellular dopamine, together with its metabolites dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) from the striatum and nucleus accumbens of anaesthetized or freely moving rats. In addition, measurements of extracellular 5-hydroxyindoleacetic acid, ascorbic acid, and uric acid were made. Basal extracellular concentrations of dopamine and DOPAC in the striatum were estimated to be 5 × 10−8M and 5 × 10−6M, respectively. d-Amphetamine (2 mg/kg s.c.) increased dopamine levels in the striatum perfusates by 14-fold, whereas levels of DOPAC and HVA decreased by 77% and 66%, respectively.