Isolation of PGP 9.5, a New Human Neurone-Specific Protein Detected by High-Resolution Two-Dimensional Electrophoresis
Article first published online: 5 OCT 2006
Journal of Neurochemistry
Volume 40, Issue 6, pages 1542–1547, June 1983
How to Cite
Doran, J. F., Jackson, P., Kynoch, P. A. M. and Thompson, R. J. (1983), Isolation of PGP 9.5, a New Human Neurone-Specific Protein Detected by High-Resolution Two-Dimensional Electrophoresis. Journal of Neurochemistry, 40: 1542–1547. doi: 10.1111/j.1471-4159.1983.tb08124.x
- Issue published online: 5 OCT 2006
- Article first published online: 5 OCT 2006
- Received September 7, 1982; accepted November 10, 1982.
- Human brain;
- Neurone-specific protein;
- Two-dimensional electrophoresis;
Protein gene product (PGP) 9.5 is a new brain-specific protein originally detected by high-resolution two-dimensional electrophoresis of the soluble proteins of human brain and other organs. We have purified this protein from human brain and raised a rabbit antihuman PGP 9.5 antiserum. The protein has a monomer molecular weight of ˜27,000 and is present in brain at concentrations at least 50 times greater than in other organs. Im-munoperoxidase labelling has localised PGP 9.5 to neurones in the human cerebral cortex with no evidence of staining of glial elements. PGP 9.5 is estimated to be present in brain at concentrations of 200-500 μg/g wet weight and represents a major protein component of neuronal cytoplasm. This new neurone-specific cytoplasmic marker may prove useful in studies of neuronal development and in the detection of neuronal damage in disease of the nervous system.