Kinetics of Transport and Phosphorylation of 2-Fluoro-2-Deoxy-d-Glucose in Rat Brain
Article first published online: 12 DEC 2006
Journal of Neurochemistry
Volume 40, Issue 1, pages 160–167, January 1983
How to Cite
Crane, P. D., Pardridge, W. M., Braun, L. D. and Oldendorf, W. H. (1983), Kinetics of Transport and Phosphorylation of 2-Fluoro-2-Deoxy-d-Glucose in Rat Brain. Journal of Neurochemistry, 40: 160–167. doi: 10.1111/j.1471-4159.1983.tb12666.x
- Issue published online: 12 DEC 2006
- Article first published online: 12 DEC 2006
- Received April 12, 1982; accepted June 22, 1982.
- Glucose transport;
- Brain glucose;
- Glucose metabolism;
- Lumped constant;
Abstract: The kinetics of transport across the blood-brain barrier and metabolism in brain (hemisphere) of [14C]2-fluoro-2-deoxy-d-glucose (FDG) were compared to that of [3H]2-deoxy-d-glucose (DG) and d-glucose in the pentobarbital-anesthetized adult rat. Saturation kinetics of transport were measured with the brain uptake index (BUI) method. The BUI for FDG was 54.3 ± 5.6. Nonlinear regression analysis gave a Km of 6.9 ± 1.1 mM and a Vmax of 1.70 ± 0.32 μmol/min/g. The K1 for glucose inhibition of FDG transport was 10.7 ± 4.4 mM. The kinetic constants of influx (k1) and efflux (K2) for FDG were calculated from the Km, Vmax, and glucose concentrations of the hemisphere and plasma (2.3 ± 0.2 μmol/g and 9.9 ± 0.4 mM, respectively). The transport coefficient (k1 FDG/k1glucose) was 1.67 ± 0.07 and the phosphorylation constant was 0.55 ± 0.16. The predicted lumped constant for FDG was 0.89, whereas the measured hexose utilization index for FDG was 0.85 ± 0.16. Conclusion: The value for the lumped constant can be predicted on the basis of the known kinetic constants of FDG and glucose transport and metabolism, as well as brain and plasma glucose levels. Knowledge of the lumped constant is crucial in interpreting data obtained from 18FDG analysis of regional glucose utilization in human brain in pathological states. We propose that the lumped constant will rise to a maximum equal to the transport coefficient for FDG under conditions of transport limitation (hypoglycemia) or elevated glycolysis (ischemia, seizures), and will fall to a minimum equal to the phosphorylation coefficient during phosphorylation limitation (extreme hyperglycemia).