Isatin: Identity with the Purified Endogenous Monoamine Oxidase Inhibitor Tribulin
Article first published online: 5 OCT 2006
Journal of Neurochemistry
Volume 51, Issue 2, pages 656–659, August 1988
How to Cite
Glover, V., Halket, J. M., Watkins, P. J., Clow, A., Goodwin, B. L. and Sandier, M. (1988), Isatin: Identity with the Purified Endogenous Monoamine Oxidase Inhibitor Tribulin. Journal of Neurochemistry, 51: 656–659. doi: 10.1111/j.1471-4159.1988.tb01089.x
- Issue published online: 5 OCT 2006
- Article first published online: 5 OCT 2006
- Received May 12, 1988; accepted May 16, 1988.
- Endogenous monoamine oxidase inhibitor;
- Endogenous benzodiazepine receptor ligand
Abstract: Purified tribulin, an endogenous monoamine oxidase (MAO) inhibitor, has been identified by direct probe insertion mass spectrometry as the indole-2,3-dione, isatin. A gas chromatographic-mass spectrometric assay for isatin has been developed and used to measure its relatively high concentrations in unpurified human urine, and in rat heart and brain. Isatin is a known compound with a broad range of biological activity; this is the first report of its presence in the animal body. Isatin is a potent inhibitor of MAO, particularly of MAO B (IC50, 3 μM), and also binds to central benzodiazepine receptors (IC50 against clonazepam, 123 μM).