Low [3H]Cytochalasin B Binding in the Cerebral Cortex of Newborn Rat
Article first published online: 5 OCT 2006
Journal of Neurochemistry
Volume 51, Issue 1, pages 206–211, July 1988
How to Cite
Morin, A. M., Dwyer, B. E., Fujikawa, D. G. and Wasterlain, C. G. (1988), Low [3H]Cytochalasin B Binding in the Cerebral Cortex of Newborn Rat. Journal of Neurochemistry, 51: 206–211. doi: 10.1111/j.1471-4159.1988.tb04857.x
- Issue published online: 5 OCT 2006
- Article first published online: 5 OCT 2006
- Received September 21, 1987; revised manuscript received January 20, 1988; accepted January 28, 1988.
- [3H]Cytochalasin B binding;
- Glucose transporter;
- Rat cerebral cortex
The concentrations of glucose transporter in the cerebral cortex and brainstem of neonatal (4–7 days old) and adult rats were measured using [3H]cytochalasin B binding. There was significantly lower binding in neonatal cortex (1.9 ± 0.7 pmol/mg protein) compared to adult (8.9 ± 2.5 pmol/mg protein). Scatchard analysis indicates this difference is due to a lower Bmax (neonate, 9.7 pmol/mg protein; adult, 18.6 ± 1.3 pmol/mg protein). Measurement of [3H]cytochalasin B binding in microvessels prepared from cortex of adult (28.1 ± 3.5 pmol/mg protein) and neonate (12.8 ± 1.9 pmol/mg protein) indicates a lower binding in the microvasculature of neonates, whereas no such difference was seen in the binding in microvessels prepared from adult and neonatal brainstem (adult, 11.8 ± 2.3 pmol/mg protein; neonate, 9.4 ± 2.7 pmol/mg protein). In both adult and neonate brain, there is an enrichment of glucose transporters in the microvasculature.